Tools for quantitiative fluorescence microscopy

ImageJ plugins

ImageJ is a versatile, open, and extendable image processing program. We developed a number of plug-ins for ImageJ to accomplish common task in fluoresence microscopy.

Blind decomposition of spectrally resolved data - single excitation

Plug-in and documentation available here: PoissonNMF

Blind decomposition of spectrally resolved data - multiple excitation

Plug-in and documentation available here: PoissonNTF

Optimize your choice of excitation wavelength and spectra channel

Plug-in and documentation available here: Optimize Channels

Library of spectra

All our plug-ins use a libray of spectra. To get started, a sample library can be download here. Unzip the file and move the folder into the ImageJ plugins folder.