PoissonNTF - fluorescence microscopy data combining multiple excitations
PoissonNTF is a ImageJ plugin, similar to
PoissonNMF, that allows to
decompose lambda stacks of fluorescently labeled samples into the contribution of
the individual dyes. In contrast to PoissonNMF, the NTF version allows to combine
spectrally resolved data acquired using different excitation wavelength.
The method is described in
Neher et al., Biophysical Journal.
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Documentation and Examples
HTML documentation
Tutorial using an example with three dyes imaged on a Leica SP2
Download
The plug-in: PoissonNTF.jar, Version 0.5.0
Authors
Richard Neher
Fabian Theis
Andre Zeug
Please address comments and question to Richard Neher and notify us of any bugs you find.
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Links and related plugins
ImageJ: rsbweb.nih.gov/ij
Linear unmixing plugin by Joachim Walter: Spectral Unmixing Plugins